|
|
 |
 |
 |
Rodney
J. Folz, M.D., Ph.D.
(Molecular
Biology and Biochemistry, Washington University, St. Louis)
Associate Professor, Dept. of Medicine
Assistant Research Professor,
Department of Cell Biology
Programs: Integrated Toxicology, Cell and Molecular Biology
|
The
major focus of my laboratory is to understand the molecular
and cellular impact oxidative stress has on a variety
of pathophysiologic processes that occur primarily in
lung, heart, and kidney. This is accomplished primarily
by genetic and molecular manipulation of antioxidant enzymes
in model systems.
Current projects
1) characterizing the human and mouse extracellular superoxide
dismutase gene;
2) using transgenic mice technology to overexpress antioxidant
genes, such as extracellular superoxide dismutase, and
studying these effects in preventing lung toxicity;
3) identifying oxidant transcriptional response elements;
4) manipulating cellular antioxidant levels using molecular
biology techniques and assessing optimal protective effects
both in vivo and in vitro;
5) developing antioxidant gene therapy for diseases of
the lung;
6) identifying markers for individuals who are at risk
for developing oxidant-induced lung injury.
Current models include environmental particulate-induced
lung injury, post bone marrow transplantation lung injury
and adult respiratory distress syndrome. Such cellular
oxidant stresses are likely to be highly localized both
at the intra and extracellular levels. The ultimate goal
of this research is to identify and understand the site-specific
and mechanism of cellular/extracellular oxidant injury.
It is hoped that knowledge derived from these studies
will allow us to genetically target or pharmacologically
enhance specific antioxidant defenses at these locations
with the end result being protection from injury. |
|
|
E-mail
rodney.folz@duke.edu
331 Medical Science Research Bldg.
Box 2620,
Duke University Medical Center
Durham, NC 27710
Telephone 919-684-3539
Fax 919-681-8936
|
Selected
Publications
Folz RJ and JD Crapo. Extracellular superoxide dismutase
(SOD3): Tissue-specific expression, genomic characterization,
and computer-assisted sequence analysis of the human EC-SOD
gene. Genomics, 22:162-171, 1994.
Folz RJ, J Guan, MF Seldin, TD Oury, JJ Enghild, and JD
Crapo. Mouse extracellular superoxide dismutase: primary
structure, tissue-specific gene expression, chromosomal
localization, and lung in situ hybridization. American
Journal of Respiratory Cell and Molecular Biology, 17:393-403,
1997.
Wilczynski SW, JJ Erasmus, WP Petros, JJ Vredenburgh,
RJ Folz. Delayed pulmonary toxicity syndrome following
high-dose chemotherapy and bone marrow transplantation
for breast cancer. American Journal of Respiratory and
Critical Care Medicine, 157:565-573, 1998.
Chen EP, Bittner HB, Davis RD, Van Trigt P, RJ Folz. Physiologic
effects of extracellular superoxide dismutase transgene
overexpression on myocardial function after ischemia and
reperfusion injury. Journal of Thoracic and Cardiovascular
Surgery, 115:450-458, 1998.
Folz RJ, A Abushamaa, and HB Suliman. EC-SOD in the airways
of transgenic mice reduces inflammation and attenuates
lung toxicity following hyperoxia. Journal of Clinical
Investigation, 103:1055-1066, 1999.
|
Folz RJ. Mechanisms of lung injury following bone marrow
transplantation. American Review of Respiratory Cell and
Molecular Biology, 20:1097-1099, 1999.
Bhalla KS, SW Wilczynski, AM Abushamaa, WP Petros, CS
McDonald, JS Loftis, NJ Chao, JJ Vredenburgh, RJ Folz.
Pulmonary toxicity of induction chemotherapy prior to
standard or high-dose chemotherapy with autologous stem
cell support. American Journal of Respiratory and Critical
Care Medicine, 161:17-25, 2000.
Folz RJ and I Nepluev. Poly A cDNA-specific (PACS) RT-PCR:
A quantitative method for the measurement of any poly
A-containing mRNA not affected by contaminating genomic
DNA. Biotechniques, 29:762-769, 2000.
Suliman HB, LK Ryan, L Bishop, RJ Folz. Prevention of
influenza-induced lung injury in transgenic mice overexpressing
extracellular superoxide dismutase. American Journal of
Physiology: Lung Cell and Molecular Physiology, 280:L69-L78,
2001.
Wright JR, P Borron, K Brinker, and RJ Folz. Surfactant
Protein A: Regulation of Innate and Adaptive Immune Responses
in Lung Inflammation. American Journal of Respiratory
Cell and Molecular Biology, in press, 2001.
Bhagat R, TA Sporn, GD Long, RJ Folz. Amiodarone and cyclophosphamide:
Potential for Enhanced Lung Toxicity. Bone Marrow Tranplantation,
in press, 2001. |
<<Back
to the top
|
|
|
