Fall courses

Biology

BIO/CBI 282/283(282 Mechanisms of Development/ 283 Developmental Genetics) - These two courses are targeted to first year graduate students in the Biological Sciences. They are taught sequentially as two half-semester minicourses, in the Fall semester. Mechanisms of development will introduce basic concepts of cell specification, morphogenesis, induction, and other mechanisms that enable cells, tissues and organs to assemble the animal. Developmental genetics will focus on genetic approaches to solve mechanistic problems of development. Coverage will focus on the use of model organisms, mainly Drosophila, C. elegans, mouse and zebrafish, in which genomics, mutational analysis, gene modifiers, epistatic relationships, gene knockouts, and transgenics have yielded important insights into the differentiation of cells and the development of complex organisms. T Th 10:55 - 12:10, 384 Nanaline Duke, McClay, Klingensmith, 2 units each.

Biochemistry

BIOCHEM 258 – Structural Biochemistry I: Structure of Macromolecules - Introduction to the principles of macromolecular protein structure and function. Examples of methods of structure determination. This course is intended to give a survey of protein structures and of how to use structural analysis to understand how proteins work. Minicourse, 1st half-semester. M W F 2:20 – 3:10.

BIOCHEM 259 – Structural Biochemistry II: Molecular Biology I - Continuation of BIOCHEM 258. Structure/function analysis of proteins as enzymes, multiple ligand binding, protein folding and stability, allostery, protein-protein interactions. Prerequisites: BIOCHEM 258, organic chemistry, physical chemistry, and introductory biochemistry. This is an introductory course to learn how to use quantitative methods to understand biological structure and function. Minicourse, 2nd half-semester. M W F 2:20 – 3:10.

BIOCHEM 267 – Molecular Genetics I: DNA and Genome Stability - Chromosome structure, replication, repair, genetic recombination, mutation and chromosome rearrangement. Molecular Genetics I & II are part of a 3 half-semester course series. This series is literature-based and covers the subject in depth. The third part (Molecular Genetics III) is taught in the spring and is listed as GENETICS 260. Minicourse, 1st half-semester. T Th 10:55 – 12:10.

BIOCHEM/UPG/CBI 268 - Biochemical Genetics II: From RNA to Protein - Mechanisms of transcription, splicing, catalytic RNA, RNA editing, mRNA stability and translation. Mini-course, 2nd half semester. Tu Th 10:05-11:20, (TBA), Steege and Staff.

Bioinformatics and Genome Technology

BGT 200/STA 270 - Statistical Methods for Computational Biology - Methods of statistical inference and stochatic modeling with application to functional genomics and computational molecular biology. Topics include: statistical theory underlying sequence analysis and database searching; Markov models; elements of Bayesian and likelihood inference; discrete data models; applied linear regression analysis; multivariate data decomposition methods (PCA, clustering); software tools for statistical computing. This course presupposes previous exposure to mathematics and statistics at the level of the BGT program prerequisites. W F 11:40-12:50, 143 Jones, Staff.

BGT 204/COMPSCI 260 - Algorithms in Computational Biology - Provides a systematic introduction to the algorithms behind the most commonly-used tools in computational biology. Surveys a wide range of methods in the field and provides a significant amount of exposure to actual tools, but primary emphasis is on understanding and analyzing the algorithms behind these tools. Introduction to common techniques in algorithmic design and analysis, including design of data structures and analysis of running time. Covers dynamic programming, string matching, probabilistic techniques, geometric algorithms, hidden Markov models, data mining, and complexity analysis. Topics explored in the context of applications of genome sequence assembly, protein and DNA homology detection, gene and promoter finding, protein structure prediction, motif identification, analysis of gene expression data, functional genomics, and phylogenetic trees. Tu Th 10:05-11:20, LSRC D106, Agarwal or Hartemink.

BGT/IMM 213S - Computational Immunology and Immunogenomics - Course will integrate empirical and computational perspectives on immunology and host defense. Students are expected to have significant preparation in either biomedicine or a quantitative science. Topics covered are intended to provide an entree into the use of computational methods for research and practice in immunology and infectious disease, from basic science to medical applications. Consent of instructor required. Tu 4:30-7:00, (TBA), Kepler and Cowell.

Cell and Molecular Biology

CMB 201 Elements of Molecular Biology and Genetics - T/Th 4:00-5:15pm; TBA; 208 CARL Bldg **Not listed in ACES

CMB 297 Modern Techniques in Molecular Biology -
This course is divided into two sections. One section deals primarily with techniques used for protein purification and analysis, and for the study of protein-protein interactions. The second deals with the molecular biology aspects, including discussions of nucleic acid sequencing and manipulation, cloning strategies, vectors, expression, hybridization and blotting methods, PCR, etc. Minicourse, 1st half-semester, MWF 9:10-10:00am; Casey;147 Nanaline Duke Bldg; 2 units.

CMB 247 Macromolecular Synthesis - The macromolecular synthesis course covers basic mechanisms of DNA synthesis, RNA transcription, protein translation, and protein stability. The material will be covered through discussion sections on both review articles (to provide adequate background) and current primary literature. Students will be expected to present data from papers covered in class and to participate in detailed discussions of those papers. Students will also write minireviews on current topics in DNA/RNA/protein synthesis. Minicourse, 2nd half-semester MWF 8-9 Kornbluth; 147 Nanaline Duke Bldg; 2 units.

Cell Biology

CBI 212 Cell & Molecular Biology of Reproduction - This course focuses on the recent cellular and molecular investigations that have dramatically advanced our understanding of reproduction. The general areas to be covered include neuroendocrinology, reproductive endocrinology, gametogenesis, and fertilization; within these topics, studies in areas such as gene regulation, intercellular communication, hormones, growth factors and signalling will be emphasized. In addition to didactic presentations, students are expected to read and present selections from the primary literature. An interactive approach is encouraged. Meeting time is arranged each year for maximum compatibility with the participants' schedules. Time: Variable Saling & Schomberg

Environment

ENVIRON 212 - Environmental Toxicology - Study of environmental contaminants from a broad perspective encompassing biochemical, ecological, and toxicological principles and methodologies. Discussion of sources, environmental transport and transformation phenomena, accumulation in biota and ecosystems. Impacts at various levels of organization, particularly biochemical and physiological effects. Prerequisites: organic chemistry and vertebrate physiology or consent of instructor. M W 11:40 AM-12:55 PM, LSRC A312, Di Giulio.

University Program in Genetics

UPG 224 Fundamentals of Human and Mouse Genetics - The genomic resources and technologies associated with the Human Genome Initiative provide opportunities for understanding and treating human genetic disease. In addition, these resources have vast implications for research in all biologic and biomedical science. This course is aimed at scientists with limited genetic background who desire a broader understanding of the concepts and tools available to the geneticist. Topics range from review of meiosis, linkage and recombination, relationships between phenotype and genotype, and inheritance patterns, to mapping of genes via genetic and physical mapping tools, to mouse models of human disease (strains and crosses, knock-outs and transgenics). The social, legal, and ethical implications of understanding genetic disease will also be reviewed. Minicourse, 1st half semester, T. Th. 9:10 - 10:25, Speer and Capel, room TBA.

UPG 225 - Readings in Human Statistical Genetics - In-depth readings of classical human statistical genetics papers that shaped the field including Morton's lod score analysis, Penrose's affected sibling pair studies, and the Elston-Stewart algorithm, among others. Student-led discussions of content. W 8:30 AM-10:00, Speer and Scott.

UPG 278 Genetics - Solutions to Biological Problems - This course provides an in-depth grounding in the use of genetic approaches to address research problems in cell and developmental biology. It begins with genetic fundamentals including dominance, linkage, complementation, types of mutants (loss of function, conditional, partial-function, dominant active & dominant negative), and genetic interactions (epistasis, suppression, synthetic lethality, intragenic complementation, nonallelic noncomplementation). There is a focus on the devising and interpretation of genetic screens, the application of reverse genetic approaches, and the use of mosaic analysis. All are covered with emphasis on how they are applied to address research problems, and are repeatedly encountered in both classical and modern molecular context (e.g. enhancer traps, PCR mutagenesis, etc.). Students gain familiarity with several major genetic model organisms, and encounter topics at the cutting edge of modern genetics. Classes are held three afternoons per week: two are socratic-style discussions and one is literature-based. 4 credits. M W 2:00- 3:45; Kiehart; 4 units.

UPG/BIO 281 - DNA, Chromosomes, and History - Past and present research on evolution, genetics, and chromosome biology. The curious path to our present understanding of inheritance including how genes got put on chromosomes and the fluctuating fortunes of DNA. Implications of current research on chromosome and genome organization for evolutionary biology. Prerequisite: an introductory course in genetics or cell or molecular biology, or consent of instructor. Tu Th 2:50-4:05, LSRC A155, Nicklas.

UPG 287/BIO 187 - Evolutionary Genetics - An introduction to the principles of evolutionary genetics, with discussion of the current literature. Levels of selection; neutral theory; variation in populations; speciation. Reconstructing evolutionary history; genomic evolution. W F 1:15-2:30, Bio Sci 144, Staff.

Immunology

IMM244 Principles of Immunology - This is a graduate level course that is open to both graduate students and advanced undergraduates. It is an introduction to the molecular and cellular basis of the immune response. Topics include anatomy of the lymphiod system, lymphocyte biology, antigen-antibody interactions, humoral and cellular effector mechanisms, and control of immune responses. The last third of the course focuses on special topics and application such as transplantation, autoimmunity, immunodeficiency, and tumor immunity. On selected days, the class is broken down into small discussion groups of approximately 15 students to discuss material introduced in the lectures or to work on problem sets. Graduate students in the Department of Immunology lead these sections. M W F 1:10 – 2:00; Dawson; 143 Jones Bldg; 3 units.

Molecular Cancer Biology

MCB/PHARM 418 - Molecular Mechanisms of Oncogenesis -
Lectures, oral presentations, and discussions on advanced topics and recent advances in the molecular biology of cancer. Particular emphasis on strategies to exploit this information in the design of intervention strategies to selectively block the growth of cancer cells. Prerequisite: Cell Biology 417. M W 10:05 AM-11:20, LSRC C335, Counter and staff.

Molecular Genetics & Microbiology

MGM/GEN 300 - Gene Regulation - Principles of prokaryotic and eukaryotic gene regulation at transcriptional and post-transcriptional levels. Topics include promoter sturcture and transcription factor function; processing, transport, and degradation of mRNA; translation. Gene regulatory pathways will be discussed. Time and place TBA; Matsunami, Nevins, Keene, Cullen, Gromeier; 3 units.

Pharmacology

PHR233 Essentials in Pharmacology & Toxicology - This is a graduate level course that is open to both graduate students and advanced undergraduates. This course will discuss in depth how drugs and toxins affect living organisms by interacting with specific receptors. The emphasis is on general principles and mechanisms rather than specific examples. Topics include: Kinetics of Drug Action; Blood-brain and placental barriers; Biotransformation; General Principles of Receptor Action; Receptor Families; Signal Transduction; Intracellular Targets Tolerance and Dependence; Discovery of New Drugs; Developing Lead Compounds; Structure-Activity Relationships; Chemical Libraries, Structure-Based Design. M W F 3:55-5:10; Slotkin; room A156 LSRC, 4 units.

Spring courses

Biochemistry

BIOCHEM 222 Structure of Biological Macromolecule - Computer graphics intensive study of some of the biological macromolecules whose three-dimensional structures have been determined at high resolution. Emphasis on the patterns and determinants of protein structure. Two-hour discussion session each week along with computer-based lessons and projects. Th 2:00 – 4:00.

BIOCHEM 291 Physical Biochemistry - Basic principles of physical chemistry as applied to biological systems. Topics include thermodynamics, kinetics, statistical mechanics, spectroscopy, and diffraction theory. Concepts discussed in the context of the biochemistry and behavior of biological macromolecules. Emphasis on quantitative understanding of biochemical phenomena, with extensive problem solving as an instructive tool. Prerequisite: undergraduate physical chemistry and one year of calculus. M W F 11:30 – 12:20.

BIOCHEM 417 Cellular Signaling - Mechanism of action of hormones at the cellular level including hormone-receptor interactions, secondary messenger systems for hormones, mechanisms of regulation of hormone responsiveness, regulation of growth, differentiation and proliferation, mechanisms of transport and ion channels, stimulus sensing and transduction. Some lectures stress the clinical correlation of the basic course concepts. M W F 8:00 – 8:50.

Bioinformatics and Genome Technology

BGT 206 - Genome Technologies -
This course introduces the laboratory and computational methodologies for genetic and protein sequencing, mapping and expression measurement. Tu Th 10:05-11:20, Sanford 224, Staff.

BGT 207/STA 277 - Computational Methods for Macromolecular Structure - This course covers concepts of modeling and computation in approaches to structure prediction in modern proteomics. It introduces advanced probabilistic models and associated inference tools and will involve custom computer programming in standard languages. M W 1:15-2:30, Perkins 421, Staff.

BGT 208/STA 278 - Gene Expression Analysis - This course covers topics spanning the technological and computational areas of modern gene expression analysis, developing computational methods in important and current problems of clinical and physiological phenotyping, including custom computation and algorithmic development. M 2:50-5:30, Languages 08, Staff.

Biology

BIO 284 - Molecular Population Genetics - Genetic mechanisms of evolutionary change at the DNA sequence level. Models of nucleotide and amino acid substitution; linkage disequilibrium and joint evolution of multiple loci; analysis of evolutionary processes, including neutrality, adaptive selection, and hitchhiking; hypothesis testing in molecular evolution; estimation of evolutionary parameters; case histories of molecular evolution. For graduate students and undergraduates with interests in genetics, evolution, or mathematics. Tu Th 8:30-9:45, Bio Sci 0018, Uyenoyama.

BIO/UPG 286 - Evolutionary Mechanisms - Population ecology and population genetics of plants and animals. Fitness concepts, life history evolution, mating systems, genetic divergence, and causes and maintenance of genetic diversity. Prerequisites: Biology 25L and 120 orequivalents. Tu Th 10:55-12:10, Bio Sci 144, Rausher and Uyenoyama.

Cell Biology

CBI 203 Introduction to Physiology - The objective of the course is to provide an introduction to Human Physiology. The course is intended for students who have not had human physiology. At the end of class, students should be able to understand how the basic organ systems (lung, cardiovascular, gastrointestinal, endocrine, and kidney) of the body work and how the systems are coordinated. M W F 9:00–10:00; Jakoi/Wright; 439 Nanaline Duke Bldg; 2 units, first half-semester.

MCB/CBI 208: Stem Cell Biology. This course is designed for first-year graduate students to learn the fundamentals of stem cell biology and to gain familiarity with current research in the field. The course will be presented in a lecture and discussion format based on the primary literature. Topics include: stem cell concepts, methodologies for stem cell research, embryonic stem cells, adult stem cells, cloning and stem cell reprogramming, and clinical applications of stem cell research. Prerequisites: undergraduate level cell biology, molecular biology, and genetics. Instructors: Tannishtha Reya, Brigid Hogan. Tentative schedule: T Th 9:00-10:30. 3 units.

CBI 296 Developmental Biology Colloquium -
Covers a broad range of problems in developmental biology and is guided by the interests of developmental biologists who will be speaking at Duke University during that particular semester. Example of topics are axis specification, embryogenesis, tissue polarity, RNA localization, axon guidance, plant developmental timing, and signaling pathways such as Notch, wingless, hedgehog. M 5:00-600 W 4:00-6:00; Klingensmith; 3 units.

University Program in Genetics

UPG 200 - Genetic Analysis for Human Disease - Quantitative and molecular aspects in the identification of human disease genes, implications for genetic counseling and risk assessment, and legal and social issues associated with the human genome initiative. Concepts of linkage analysis in Mendelian and complex disease, molecular approaches to disease gene cloning, molecular mechanisms of disease gene expression, gene therapy, and the utility of animal models for understanding human disease. Prerequisite: University Program in Genetics 278 or equivalent, and graduate status or consent of instructor. W 10:30-12:00, LSRC B102, Marchuk.

UPG 222 Genetic Analysis of Cellular Function -
This class uses studies of fungi as a basis to discuss genetic approaches to cell biological problems. Topics covered include signal transduction, cell cycle, nutrient sensing, prions and genomics. 2-3 papers are assigned for each class and the class is driven by in-depth, critical discussion of these papers T Th 4:00-5:30; Heitman and Lew; 3 units.

Molecular Genetics & Microbiology

MGM/UPG 232 - Human Genetics - Topics include segregation, genetic linkage, population genetics, multifactorial inheritance, biochemical genetics, cytogenetics, somatic cell genetics, neurogenetics, cancer genetics, clinical genetics, positional cloning, complex disease. Lectures plus weekly discussion of assigned papers from the research literature. Prerequisites: University Program in Genetics 278 or equivalent, and graduate status or consent of instructor. Tu Th 8:30 AM-9:45, (TBA), Marchuk, Pericak-Vance, and Speer.

MGM 252 General Virology and Viral Oncology -
(Summary [Graduate core course]) - This course explores the molecular biology of mammalian viruses, with major emphasis on mechanisms of virus replication, virus-host-interactions, viral pathogenicity, and the relationships between virus infections and cancers. M W F 10:30 -11:20; Keene; 418 Jones Bldg; 3 units.

MGM 282 Microbial Pathogenesis - (Summary [Graduate Core Course]) Modern molecular genetic approaches to understanding the pathogenic bacteria and fungi. The course also examines underlying mechanisms of pathogenesis and host- parasite relationships that contribute to the infectious disease process. M W F 3:55 - 4:45; Kreuzer & McCusker; 418 Jones Bldg; 3 units.